Validation
| Gene Symbol | Curated Descriptions | Cell Lines | Isolations | Validations | Control-case |
Tumor Stage & Subtype |
Clinical Use | PMID |
|---|---|---|---|---|---|---|---|---|
| RPPH1 | RPPH1 distinguish exosomes produced by low metastatic breast cancer cell line (MDA-MB-436) from that produced by highly metastatic breast cancer cell line (MDA-MB-231) | MDA-MB-436 ,MDA-MB-231 | centrifugation | transmission electron microscopy | 24255815 | |||
| S1PR2 | S1P2 is released from breast cancer cells in exosomes and is processed by fibroblasts to promote ERK signaling and proliferation of breast cancer cells | MDA-MB-231 | ultracentrifugation | electron microscopy | 30034630 | |||
| TGFBR1 | Presumably aid in targeting the primary cancer cells to specific metastatic sites | 4T1 | ultracentrifugation | transmission electron microscopy | metastasis | 29115712 | ||
| TSG101 | Tumour-derived exosomes interfere with the tumour-specific function of immune cells and constitute an additional mechanism how tumours escape from immune surveillance | BT474 | ultracentrifugation | electron microscopy | 21293856 | |||
| UCHL1 | UCH-L1-containing exosomes can transfer chemoresistance to recipient cells and these exosomes may be useful as non-invasive diagnostic biomarkers for detection of chemoresitance in breast cancer patients, achieving more effective and individualized chemotherapy | MCF7 | ultracentrifugation | transmission electron microscopy | chemotherapy | 28334432 | ||
| CD82 | CD82 expression in exosomes from breast cancer samples is a sensitive biomarker | centrifugation | western blot | 80 breast cancer patients (case) 80 patients with benign breast diseases (control) | stage 0 to stage IV | early diagnosis | 30604894 | |
| BIRC5 | Differential expression of exosomal-Survivin, particularly Survivin-2B, may serve as a diagnostic and/or prognostic marker | ExoQuick kit | laser-scanning confocal microscopy | 40 breast cancer samples (case) 10 healthy samples (control) | staging I-IV breast cancer | early diagnostic | 24620748 | |
| hsa-miR-223-3p | Exosomal miR-223-3p may be a useful preoperative biomarker to identify the invasive lesions of DCIS patients diagnosed by biopsy | MCF7 | ultracentrifugation | electron microscope | 175 breast cancer patients (case) 3 healthy controls (control) | ductal carcinoma in?situ (DCIS) breast cancer? | early diagnosis | 29805680 |
| ADAM10 | The mature ADAM10 in exosomes and its proteolytic activity is regulated by tetraspanins CD9, CD81, CD82, which was associated with breast cancer | electron microscopy,flow cytometry | 32 breast cancer patients (case) 30 healthy females (control) | ER and/or PR +/- and HER2+/ -,triple-negative breast cancer,stages Il and III breast cancer | 30678441 | |||
| hsa-let-7a | Hsa-let-7a might be considered as a respectable diagnostic tool for monitoring of breast cancer patients | centrifugation ultracentrifugation and filtration or with the Exoquick-TC reagent (System Biosciences,Mountain View,CA,USA) | electron microscopy,nanoparticle tracking analysis,and western blot | 16 breast cancer plasma samples (case) 16 healthy plasma samples (control) | ER+/-,PR+/-,HER2+/- stages II, III breast cancer | 27608715 |