Validation
| Gene Symbol | Curated Descriptions | Cell Lines | Isolations | Validations | Control-case | Tumor Stage & Subtype | PMID |
|---|---|---|---|---|---|---|---|
| hsa-miR-21 | Hsa-miR-21 has significantly up-regulated and has been extensively implicated in cancer progression | MCF7,MDA-MB-2314T1 | ultracentrifugation | transmission electron microscopy | 11 breast cancer samples (case) 8 healthy samples (control) | triple negative metastatic breast carcinoma | 25446899 |
| hsa-miR-27a | Hsa-miR-27a has significantly up-regulated and has been extensively implicated in cancer progression | MCF7,MDA-MB-2314T1 | ultracentrifugation | transmission electron microscopy | 11 breast cancer samples (case) 8 healthy samples (control) | triple negative metastatic breast carcinoma | 25446899 |
| hsa-miR-373 | Hsa-miR-373 has significantly up-regulated and has been extensively implicated in cancer progression | MCF7,MDA-MB-231,4T1 | ultracentrifugation | transmission electron microscopy | 11 breast cancer samples (case) 8 healthy samples (control) | triple negative metastatic breast carcinoma | 25446899 |
| hsa-miR-103 | Preliminary data indicated that detection of cancer-specific miRNA (miR-103, miR-191, miR-195) in exosomes associated with the fraction of red blood cells allowed to discriminate HFs and BCPs more precisely compared to cell-free exosomes circulating in plasma | centrifugation | transmission electron microscopy | 10 patients (case) 10 healthy donors (control) | 30868953 | ||
| TSG101 | Tumour-derived exosomes interfere with the tumour-specific function of immune cells and constitute an additional mechanism how tumours escape from immune surveillance | BT474 | ultracentrifugation | electron microscopy | 21293856 | ||
| hsa-miR-223 | MiR-223 promoted the invasion of breast cancer cells via the Mef2c-β-catenin pathway | MDA-MB-231 | ultracentrifugation | electronic microscopy | 21939504 | ||
| hsa-miR-31 | Hsa-miR-31 modulates MCF-7 cells growth by specially targeting the histone deacetylase 2 | MCF7 | centrifugation | transmission electron microscopy | 30916359 | ||
| hsa-miR-34a | MCF-Exo also contains mir-34a, which regulates several genes including p53 | MCF7,MDA-MB231 | ultracentrifugation | electron microscopy | 24468161 | ||
| hsa-miR-720 | Hsa-miR-720 is characterized by excessive release from breast cancer cells while retaining relatively low concentrations of miRNA in the cells and could be used to detect the presence of malignant cells in the body | MDA-MB-231,MCF7 | centrifugation | negative-staining electron microscopy | 22772984 | ||
| RPL28 | RPL28 distinguish exosomes produced by low metastatic breast cancer cell line (MDA-MB-436) from that produced by highly metastatic breast cancer cell line (MDA-MB-231) | MDA-MB-436 ,MDA-MB-231 | centrifugation | transmission electron microscopy | 24255815 |