Validation
Gene Symbol |
Curated Descriptions | Cell Lines | Isolations | Validations | Control-case | Tumor Stage & Subtype | Clinical Use | PMID |
|---|---|---|---|---|---|---|---|---|
| HER2 | The exosomal HER2 expression levels were almost consistent with that in tumor tissues assessed by immunohistochemical staining | MCF7,MDA-MB-231 | centrifugation | transmission electron microscopy | 19 breast cancer patients (case) | early diagnosis | 28369094 | |
| HOXC6 | Stemness markers and enhance proliferation,migration and invasion abilities of neighboring cells | MDA-MB-231,T47D | centrifugationand filtration | transmission electron microscopy | metastasis | 26528758 | ||
| hsa-let-7a | Hsa-let-7a might be considered as a respectable diagnostic tool for monitoring of breast cancer patients | centrifugation ultracentrifugation and filtration or with the Exoquick-TC reagent (System Biosciences,Mountain View,CA,USA) | electron microscopy,nanoparticle tracking analysis,and western blot | 16 breast cancer plasma samples (case) 16 healthy plasma samples (control) | ER+/-,PR+/-,HER2+/- stages II, III breast cancer | 27608715 | ||
| hsa-miR-100 | Target gene prediction and pathway analysis showed the involvement of miR-100, miR-222, and miR-30a in pathways implicated in cancer pathogenesis, membrane vesiculation and therapy failure，the transmition of chemoresistance by a horizontal transfer of miRNAs | MCF7 | ultracentrifugation | transmission electron microscopy | drug resistance | 24740415 | ||
| hsa-miR-101 | In conclusion, our findings suggest cell-free miR-101 and miR-373 as breast cancer-specific markers | MCF7,MDA-MB-231 | ExoQuick exosome precipitation solution (BioCat,Heidelberg,Germany) | western blot (BioCat),BD Biosciences and Axiovert 200 microscope | 50 breast cancer samples (case) 12 healthy samples (control) | luminal,HER2(+),TN | 25333260 | |
| hsa-miR-103 | Preliminary data indicated that detection of cancer-specific miRNA (miR-103, miR-191, miR-195) in exosomes associated with the fraction of red blood cells allowed to discriminate HFs and BCPs more precisely compared to cell-free exosomes circulating in plasma | centrifugation | transmission electron microscopy | 10 patients (case) 10 healthy donors (control) | 30868953 | |||
| hsa-miR-105 | Hsa-miR-105 has significantly up-regulated and has been extensively implicated in cancer progression | MDA-MB-231 | ultracentrifugation | electron microscopy | metastasis | 24735924 | ||
| hsa-miR-105 | Higher levels of exosomal miRNA-21, miRNA-222, and miRNA-155 were significantly associated with the presence of circulating tumor cells;Liquid biopsies based on exosomal miRNAs and circulating tumor cells can be a complementary clinical tool for improving breast cancer diagnosis and prognosis | fluorescence microscope | 53 breast cancer women (case) 8 healthy donors (control) | localized breast cancer | neoadjuvant chemotherapy | 30728048 | ||
| hsa-miR-106b | The mir-106b is found in higher levels in MCF-Eox as well, which can promote breast cancer invasion and metastasis by targeting BRMS1 and RB | MCF7,MDA-MB231 | ultracentrifugation | electron microscopy | 24468161 | |||
| hsa-miR-10a | Hsa-miR-10a has significantly up-regulated and has been extensively implicated in cancer progression | MCF7,MDA-MB-2314T1 | ultracentrifugation | transmission electron microscopy | 11 breast cancer samples (case) 8 healthy samples (control) | triple negative metastatic breast carcinoma | 25446899 |