Validation
| Gene Symbol | Curated Descriptions | Cell Lines |
Isolations |
Validations | Control-case | Tumor Stage & Subtype | Clinical Use | PMID |
|---|---|---|---|---|---|---|---|---|
| hsa-miR-10b | Hsa-miR-10b secretes protein levels that inhibit its target genes such as HOXD10 and KLF4 and induces invasion of breast cancer cells | MDA-MB-231 | centrifugation | scanning electron and confocal microscopy | metastasis | 25428807 | ||
| EEF1A1 | EEF1A1 distinguish exosomes produced by low metastatic breast cancer cell line (MDA-MB-436) from that produced by highly metastatic breast cancer cell line (MDA-MB-231) | MDA-MB-436 ,MDA-MB-231 | centrifugation | transmission electron microscopy | 24255815 | |||
| hsa-miR-195 | Preliminary data indicated that detection of cancer-specific miRNA (miR-103, miR-191, miR-195) in exosomes associated with the fraction of red blood cells allowed to discriminate HFs and BCPs more precisely compared to cell-free exosomes circulating in plasma | centrifugation | transmission electron microscopy | 10 patients (case) 10 healthy donors (control) | 30868953 | |||
| hsa-miR-191 | Preliminary data indicated that detection of cancer-specific miRNA (miR-103, miR-191, miR-195) in exosomes associated with the fraction of red blood cells allowed to discriminate HFs and BCPs more precisely compared to cell-free exosomes circulating in plasma | centrifugation | transmission electron microscopy | 10 patients (case) 10 healthy donors (control) | 30868953 | |||
| hsa-let-7a | Hsa-let-7a might be considered as a respectable diagnostic tool for monitoring of breast cancer patients | centrifugation ultracentrifugation and filtration or with the Exoquick-TC reagent (System Biosciences,Mountain View,CA,USA) | electron microscopy,nanoparticle tracking analysis,and western blot | 16 breast cancer plasma samples (case) 16 healthy plasma samples (control) | ER+/-,PR+/-,HER2+/- stages II, III breast cancer | 27608715 | ||
| hsa-miR-21 | Based on the selective enrichment and absolute abundance, we identified miR-1246, miR-122, miR-21, and let-7a as candidate exosome microRNAs that may serve as biomarkers indicative of breast cancer | centrifugation ultracentrifugation and filtration or with the Exoquick-TC reagent (System Biosciences,Mountain View,CA,USA) | electron microscopy,nanoparticle tracking analysis,and western blot | 16 breast cancer plasma samples (case) 16 healthy plasma samples (control) | ER+/-,PR+/-,HER2+/- stages II, III breast cancer | 27608715 | ||
| HTR7 | Stemness markers and enhance proliferation,migration and invasion abilities of neighboring cells | MDA-MB-231,T47D | centrifugationand filtration | transmission electron microscopy | metastasis | 26528758 | ||
| NANOG | Stemness markers and enhance proliferation,migration and invasion abilities of neighboring cells | MDA-MB-231,T47D | centrifugationand filtration | transmission electron microscopy | metastasis | 26528758 | ||
| KISS1R | Stemness markers and enhance proliferation,migration and invasion abilities of neighboring cells | MDA-MB-231,T47D | centrifugationand filtration | transmission electron microscopy | metastasis | 26528758 | ||
| HOXC6 | Stemness markers and enhance proliferation,migration and invasion abilities of neighboring cells | MDA-MB-231,T47D | centrifugationand filtration | transmission electron microscopy | metastasis | 26528758 |