Validation
| Gene Symbol | Curated Descriptions |
Cell Lines |
Isolations | Validations | Control-case | Tumor Stage & Subtype | Clinical Use | PMID |
|---|---|---|---|---|---|---|---|---|
| Del-1 | As the current prospective cohort study demonstrated a normalization of exosomal Del-1 after curative surgery, exosomal Del-1 can be confirmed as a potent diagnostic biomarker for breast cancer. Plus, a high Del-1 level after surgery seems related with early relapse suggesting a potential prognostic marker by identifying the existence of residual tumor | ultracentrifugation | transmission electron microscopy | 114 breast cancer patients (case) | diagnosis | Link | ||
| hsa-miR-105 | Higher levels of exosomal miRNA-21, miRNA-222, and miRNA-155 were significantly associated with the presence of circulating tumor cells;Liquid biopsies based on exosomal miRNAs and circulating tumor cells can be a complementary clinical tool for improving breast cancer diagnosis and prognosis | fluorescence microscope | 53 breast cancer women (case) 8 healthy donors (control) | localized breast cancer | neoadjuvant chemotherapy | 30728048 | ||
| hsa-miR-382 | Our study showed that serum-circulating miR-145, miR-155, and miR-382 are present in exosomes. These miRNAs were previously proposed as noninvasive biomarkers to distinguish between BC patients and healthy individuals | ExoQuick exosome precipitation solution | scanning electron Microscopy,atomic force microscopy, anoparticle tracking analysis | 20 diagnosis of ductal carcinoma (case) 5 healthy donors (control) | stages I, III, and IV breast cancer | 30891102 | ||
| ADAM10 | The mature ADAM10 in exosomes and its proteolytic activity is regulated by tetraspanins CD9, CD81, CD82, which was associated with breast cancer | electron microscopy,flow cytometry | 32 breast cancer patients (case) 30 healthy females (control) | ER and/or PR +/- and HER2+/ -,triple-negative breast cancer,stages Il and III breast cancer | 30678441 | |||
| BIRC5 | Differential expression of exosomal-Survivin, particularly Survivin-2B, may serve as a diagnostic and/or prognostic marker | ExoQuick kit | laser-scanning confocal microscopy | 40 breast cancer samples (case) 10 healthy samples (control) | staging I-IV breast cancer | early diagnostic | 24620748 | |
| CD24 | We suggest that CD24 could be an additional marker for the enrichment of tumor-derived exosomes from blood | AllPrep DNA/RNA Mini Kit (Qiagen,Hilden,Germany) centrifugation | electron microscopy | 8 breast cancer samples (case) 6 Lung cancer patients (control) | 21601258 | |||
| hsa-miR-222 | Higher levels of exosomal miRNA-21, miRNA-222, and miRNA-155 were significantly associated with the presence of circulating tumor cells;Liquid biopsies based on exosomal miRNAs and circulating tumor cells can be a complementary clinical tool for improving breast cancer diagnosis and prognosis | fluorescence microscope | 53 breast cancer women (case) 8 healthy donors (control) | localized breast cancer | neoadjuvant chemotherapy | 30728048 | ||
| hsa-miR-21 | Higher levels of exosomal miRNA-21, miRNA-222, and miRNA-155 were significantly associated with the presence of circulating tumor cells;Liquid biopsies based on exosomal miRNAs and circulating tumor cells can be a complementary clinical tool for improving breast cancer diagnosis and prognosis | fluorescence microscope | 53 breast cancer women (case) 8 healthy donors (control) | localized breast cancer | neoadjuvant chemotherapy | 30728048 | ||
| hsa-miR-195 | Preliminary data indicated that detection of cancer-specific miRNA (miR-103, miR-191, miR-195) in exosomes associated with the fraction of red blood cells allowed to discriminate HFs and BCPs more precisely compared to cell-free exosomes circulating in plasma | centrifugation | transmission electron microscopy | 10 patients (case) 10 healthy donors (control) | 30868953 | |||
| hsa-miR-191 | Preliminary data indicated that detection of cancer-specific miRNA (miR-103, miR-191, miR-195) in exosomes associated with the fraction of red blood cells allowed to discriminate HFs and BCPs more precisely compared to cell-free exosomes circulating in plasma | centrifugation | transmission electron microscopy | 10 patients (case) 10 healthy donors (control) | 30868953 |