Validation
| Gene Symbol | Curated Descriptions | Cell Lines | Isolations |
Validations |
Control-case | Tumor Stage & Subtype | Clinical Use | PMID |
|---|---|---|---|---|---|---|---|---|
| hsa-miR-301a | MCF-Exo contains a higher amount of hsa-miR-301a,and overexpression has been considered a negative prognostic indicator in lymph node-negative (LNN) invasive ductal breast cancer | MCF7,MDA-MB231 | ultracentrifugation | electron microscopy | metastasis | 24468161 | ||
| hsa-miR-130a | Comparing the miRNAs in MDA-Exo to MCF-Exo showed a higher expression of tumorigenic mir-130a in MDA-Exo. it has been shown that mir-130a contribute to tumorigenesis of colon cancer by regulating TGB-β/Smad signaling | MCF7,MDA-MB231 | ultracentrifugation | electron microscopy | 24468161 | |||
| hsa-miR-106b | The mir-106b is found in higher levels in MCF-Eox as well, which can promote breast cancer invasion and metastasis by targeting BRMS1 and RB | MCF7,MDA-MB231 | ultracentrifugation | electron microscopy | 24468161 | |||
| hsa-miR-105 | Hsa-miR-105 has significantly up-regulated and has been extensively implicated in cancer progression | MDA-MB-231 | ultracentrifugation | electron microscopy | metastasis | 24735924 | ||
| CD24 | We suggest that CD24 could be an additional marker for the enrichment of tumor-derived exosomes from blood | AllPrep DNA/RNA Mini Kit (Qiagen,Hilden,Germany) centrifugation | electron microscopy | 8 breast cancer samples (case) 6 Lung cancer patients (control) | 21601258 | |||
| RN7SL1 | Unshielded RN7SL1 in exosomes acts as a DAMP and activates breast cancer RIG-I to promote aggressive features of cancer | centrifugation | electron microscopy | metastasis and therapy resistance | 28709002 | |||
| hsa-miR-34a | MCF-Exo also contains mir-34a, which regulates several genes including p53 | MCF7,MDA-MB231 | ultracentrifugation | electron microscopy | 24468161 | |||
| CD47 | A differential CD47 expression in blood-derived individual circulating exosomes that is correlated with breast cancer status, demonstrating a great potential of individual exosome profiles in biomarker discovery | MDA-MB-231,MCF-12A | ultracentrifugation | electron microscopy | 50 breast cancer samples (case) 40 healthy samples (control) | diagnosis | 27819324 | |
| hsa-miR-503 | Our data are the first to reveal the involvement of the endothelium in the modulation of tumor development via the secretion of circulating miR-503 in response to chemotherapy treatment | MDA-MB-231 | ultracentrifugation | electron microscopy | 29 breast cancer samples (case) | primary breast cancer | neoadjuvant chemotherapy | 25860935 |
| S1PR2 | S1P2 is released from breast cancer cells in exosomes and is processed by fibroblasts to promote ERK signaling and proliferation of breast cancer cells | MDA-MB-231 | ultracentrifugation | electron microscopy | 30034630 |