Validation
Gene Symbol |
Curated Descriptions | Cell Lines | Isolations | Validations | Control-case | Tumor Stage & Subtype | Clinical Use | PMID |
|---|---|---|---|---|---|---|---|---|
| hsa-miR-338-3p | Of 384 miRNAs, three miRNAs (miR-338-3p, miR-340-5p, and miR-124-3p) were significantly upregulated and eight (miR-29b-3p, miR-20b-5p, miR-17-5p, miR-130a-3p, miR-18a-5p, miR-195-5p, miR-486-5p, and miR-93-5p) were significantly downregulated in the patients with recurrence | ExoQuick | nanosight LM10 instrumen western blottingt | 16 breast cancer patients with recurrence (case) 16 breast cancer patients without recurrence (control) | Luminal (ER and/or PR + and HER2 -),HER2 (ER and/or PR +/- and HER2 +),TN (ER and PR and HER2 -) | recurrence | 29050253 | |
| hsa-miR-340-5p | Of 384 miRNAs, three miRNAs (miR-338-3p, miR-340-5p, and miR-124-3p) were significantly upregulated and eight (miR-29b-3p, miR-20b-5p, miR-17-5p, miR-130a-3p, miR-18a-5p, miR-195-5p, miR-486-5p, and miR-93-5p) were significantly downregulated in the patients with recurrence | ExoQuick | nanosight LM10 instrumen western blottingt | 16 breast cancer patients with recurrence (case) 16 breast cancer patients without recurrence (control) | Luminal (ER and/or PR + and HER2 -),HER2 (ER and/or PR +/- and HER2 +),TN (ER and PR and HER2 -) | recurrence | 29050253 | |
| hsa-miR-34a | MCF-Exo also contains mir-34a, which regulates several genes including p53 | MCF7,MDA-MB231 | ultracentrifugation | electron microscopy | 24468161 | |||
| hsa-miR-372 | The levels of exosomal miR-101 and miR-372 were tumor-specifically increased and higher in breast cancer patients than in healthy women | MCF7,MDA-MB-231 | ExoQuick exosome precipitation solution (BioCat,Heidelberg,Germany) | western blot (BioCat),BD Biosciences and Axiovert 200 microscope | 50 breast cancer samples (case) 12 healthy samples (control) | luminal,HER2(+),TN | 25333260 | |
| hsa-miR-373 | Hsa-miR-373 has significantly up-regulated and has been extensively implicated in cancer progression | MCF7,MDA-MB-231,4T1 | ultracentrifugation | transmission electron microscopy | 11 breast cancer samples (case) 8 healthy samples (control) | triple negative metastatic breast carcinoma | 25446899 | |
| hsa-miR-373 | In conclusion, our findings suggest cell-free miR-101 and miR-373 as breast cancer-specific markers | MCF7,MDA-MB-231 | ExoQuick exosome precipitation solution (BioCat,Heidelberg,Germany) | western blot (BioCat),BD Biosciences and Axiovert 200 microscope | 50 breast cancer samples (case) 12 healthy samples (control) | luminal,HER2(+),TN | 25333260 | |
| hsa-miR-382 | Our study showed that serum-circulating miR-145, miR-155, and miR-382 are present in exosomes. These miRNAs were previously proposed as noninvasive biomarkers to distinguish between BC patients and healthy individuals | ExoQuick exosome precipitation solution | scanning electron Microscopy,atomic force microscopy, anoparticle tracking analysis | 20 diagnosis of ductal carcinoma (case) 5 healthy donors (control) | stages I, III, and IV breast cancer | 30891102 | ||
| hsa-miR-486-5p | Of 384 miRNAs, three miRNAs (miR-338-3p, miR-340-5p, and miR-124-3p) were significantly upregulated and eight (miR-29b-3p, miR-20b-5p, miR-17-5p, miR-130a-3p, miR-18a-5p, miR-195-5p, miR-486-5p, and miR-93-5p) were significantly downregulated in the patients with recurrence | ExoQuick | nanosight LM10 instrumen western blottingt | 16 breast cancer patients with recurrence-16 breast cancer patients without recurrence | Luminal (ER and/or PR + and HER2 -),HER2 (ER and/or PR +/- and HER2 +),TN (ER and PR and HER2 -) | recurrence | 29050253 | |
| hsa-miR-503 | Our data are the first to reveal the involvement of the endothelium in the modulation of tumor development via the secretion of circulating miR-503 in response to chemotherapy treatment | MDA-MB-231 | ultracentrifugation | electron microscopy | 29 breast cancer samples (case) | primary breast cancer | neoadjuvant chemotherapy | 25860935 |
| hsa-miR-720 | Hsa-miR-720 is characterized by excessive release from breast cancer cells while retaining relatively low concentrations of miRNA in the cells and could be used to detect the presence of malignant cells in the body | MDA-MB-231,MCF7 | centrifugation | negative-staining electron microscopy | 22772984 |